Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS propane BioXtra 925G
REAGENT BIS-TRIS propane BioXtra 925G
CARTERRA INC 0.1 M MES PH 5.5
NC2232564 0.1 M MES PH 5.5
Research Products International Corp PIPES Sesquisdm Salt 250G
PIPES Sesquisodium Salt, also known as PIPES Na3, is a chemical compound that has significant applications in the life science marketplace. PIPES stands for piperazine-N,N'-bis(2-ethanesulfonic acid), and the sesquisodium salt refers to the trisodium salt form of PIPES.
It is primarily utilized as a buffering agent. Buffers are substances that help maintain a stable pH in biological and biochemical processes, ensuring optimal conditions for various enzymatic reactions and cellular activities.
PIPES Sesquisodium Salt is particularly valuable in biological research, molecular biology, and cell culture applications. It offers excellent buffering capacity in the physiological pH range (6.1 - 7.5), making it suitable for a wide range of experimental conditions.
Research Products International Corp PIPES Sodium Salt 500 G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Research Products International Corp Phosphate Buffered Saline, 5 L
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
Alkali Scientific QuickSilver TBS-T Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 500g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 500g
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 25g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 25g
INTACT GENOMICS INC IG Autoinduction Medium, 5 x 1L
Intact Genomics (IG) Autoinduction Medium promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins without time-consuming steps such as monitoring optical density (OD) or adding induction components like IPTG. Unlike traditional LB-IPTG induction systems, IG Autoinduction Medium allows regulated protein expression in any expression system that is solely inducible by IPTG from E. coli strains containing a fully functional lac operon.
Sigma Aldrich Fine Chemicals Biosciences MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | 1132-61-2 | MFCD00006183 | 6X500G
MOPS BioPerformance Certified, suitable for cell culture, >=99.5% (titration) | Purity: >=99.5% (titration) | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 6X500G
Research Products International Corp MES Sodium Salt 5 KG
MES Sodium Salt, also known as 2-(N-Morpholino)ethanesulfonic acid sodium salt, is a zwitterionic buffer commonly used in biological and biochemical research.
In the life science field, MES Sodium Salt is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 5.5 to 6.7, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MES Sodium Salt is commonly used in protein purification, enzyme assays, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MES Sodium Salt is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes.;
Research Products International Corp MES Anhydrous 5 KG
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp BES 500 G
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8